NL5+ LINE SCAN CONFOCAL
Confocal NL NL5+The NL5+ is the second generation of fast line-scanning confocal technologies. It provides high-contrast images from thicker specimens such as organoids, tissue samples, plant and animal model organisms. The gentle conditions for your live samples allow the NL5+ to excel in long time-lapse experiments and imaging low-signal samples.
The combination of camera-based detection and a set of filters allows you to acquire your images in up to six different colors. This gives you an improved temporal resolution, high sensitivity and unprecedented signal-to-noise ratio. You get even faster imaging with cleaner results, thanks to additional motorized single-band emission filters.
Various system configurations are available for your specific application needs.
Contact us for advice.
Or visit the Confocal nl website: Confocal nl NL5+
3D live cell imaging with multiple filters
The NL5+ series takes the best of two worlds; point scanners and spinning disc, leading to a system with options to do confocal, widefield or brightfield observations.
Phototoxicity is a central problem in the application of confocal microscopy for live cell imaging. To overcome these challenges, we developed the second generation of fast line-scanning confocal technologies. Using a line scanner instead of a point scanner, a confocal microscope provides high temporal resolution, at the same time reducing the phototoxicity and allowing long-term imaging. A slit pinhole, together with an extremely sensitive sCMOS camera as a detector, makes the NL5+ series the perfect tool for fast and deep 3D live cell imaging.
No pinhole crosstalk
Contrary to spinning disk systems, another advantage of the NL5+ series and its slit pinhole is that it does not lead to pinhole crosstalk when imaging deep within the sample. The NL5+ series reaches 240 nm lateral resolution, which can be easily converted into 170 nm with on-the-fly deconvolution.
NL5+ is an excellent add-on to any research microscope. It allows converting any widefield system into a fast scanning confocal with high resolution and sensitivity.
The impact of an add-on filter
The NL5+ is a confocal system which enables even faster imaging with cleaner results. This is a direct result of one of the new functionalities: its additional motorized filter wheel.
Our system uses a line scanner, instead of a point scanner confocal microscope. This provides high temporal resolution, while at the same time reducing phototoxicity. Since phototoxicity is one of the biggest challenges for researchers, this makes the NL5+ the perfect tool for live cell imaging.
You can also add the NL5+ to any widefield microscope. This will turn it into an advanced fast-scanning confocal imaging system.
| Specifications | |
| Detector | Camera (sCMOS) |
| Resolution in real time | 170 nm deconvolved, 240 nm raw |
| Detector sensitivity | Up to 95% QE |
| FOV | FOV 18mm (FN18); 330×330 µm (40x objective) |
| Speed in line scanning | up to 75 fps for 2048×512 px and up to 50 fps for 2048×2048 px |
| Speed in fixed line mode | >1000 fps and more (camera limited) |
| Wavelength | Standard VIS (400-700 nm) |
| Software | KRONOS, ZEN, Micromanager, SDK available for integration on request |
| Deconvolution | SVI Huygens (post processing) |
| Modalities | Brightfield, Widefield and Confocal (motorized) |
| Emission filters | 6 or 7 positions |
| Weight | 4.5kg |
Various system configurations are available for your specific application needs.
Contact us for advice.
Or visit the Confocal nl website: Confocal nl NL5+
